SmartBatch+: New high-throughput system for rapid active tissue clearing and immunolabeling.
SmartBatch+ combines active tissue clearing, leveraging our new
Clear+ system, and active immunolabeling into one turn-key batch tissue processing device! Now you can clear and label up to 12 whole mouse brains in just one day.
An advance on our founder’s CLARITY method, Clear+ tissue clearing provides maximum optical transparency with no tissue expansion; eFLASH and patented SE technologies then uniformly label whole
organs from surface to core.
FEATURES & HIGHLIGHTS
Multi-functional: SmartBatch+ switches between clearing and labeling modes simply by changing buffers.
Fast, uniform labeling: Our system uses eFLASH technology (bioRxiv 2019), combining SWITCH (Cell 2015) with our patented stochastic electrotransport (SE, PNAS 2015) method to uniformly label intact tissue volumes.
Cost-effective: SmartBatch+ uses just 4-20 μg of antibody pertarget to label a sample the size of a whole mouse brain, and requires <12 mins hands-on time per sample.
High-throughput: Our device can actively clear or label up to 12 whole mouse brains, 2 whole rat brains, or comparably sized samples per batch.
Ideal for light sheet imaging: Combined with SHIELD tissue preservation, SmartBatch+ protects fluorescent protein signal, antigenicity, and molecular & physical architecture through all tissue processing steps.
TISSUE CLEARING
Combining electrophoretic clearing with our Clear+ tissue clearing method, a rotating electric field drives charged SDS micelles into samples for quick and uniform delipidation. Clear+ is the only known technique to produce no change in sample morphology after clearing. Compatible with different tissue types and large intact samples, SmartBatch+ clearing takes minutes to set up and 1 day to complete.
Before delipidation After index-matching After immunolabeling
IMMUNOLABELING
Recently published advances on our patented SE method enable antibody diffusion without surface saturation and uniform labeling throughout intact volumes. SmartBatch+ also uses an order of magnitude less antibody than passive clearing methods such as iDISCO and CUBIC.
To date, we have validated and optimized >50 antibodies, providing coverage of popular targets related to neuroscience, cancer, development, and disease.
OUR OPTIMIZED TISSUE
PROCESSING PIPELINE:
Exceptional tissue preservation with SHIELD
High-throughput, active tissue clearing using our Clear+ system with SmartBatch+
Active immunolabeling with SmartBatch+
Refractive index-matching with EasyIndex to optically clear samples
Rapid, high resolution light sheet imaging with SmartSPIM
Publication-quality data analysis outputs with SmartAnalytics
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